Transformation of Candida albicans with a synthetic hygromycin B resistance gene
نویسندگان
چکیده
منابع مشابه
Single allele knock-out of Candida albicans CGT1 leads to unexpected resistance to hygromycin B and elevated temperature.
Almost all eukaryotic mRNAs are capped at their 5'-terminus. Capping is crucial for stability, processing, nuclear export and efficient translation of mRNA. We studied the phenotypic effects elicited by depleting a Candida albicans strain of mRNA 5'-guanylyltransferase (mRNA capping enzyme; CGT1). Construction of a Cgt1-deficient mutant was achieved by URA-blaster-mediated genetic disruption of...
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ژورنال
عنوان ژورنال: Yeast
سال: 2010
ISSN: 0749-503X
DOI: 10.1002/yea.1813